Animal and Plant Health Inspection Service | Veterinary Services | 1
1. Disease Information
1.1 General Disease and Pathogen Information: Varroosis is infestation by the
ectoparasitic mite, Varroa destructor, of brood and adult honey bees, Apis mellifera.
The mites are found throughout the world due to the global scale of honey bee trade
and difficulty in detection. Nearly all honey bee colonies around the world are infested
with Varroa to some degree. Varroa infests late-stage larval honey bees just before the
brood cell is sealed for pupation. In the pupal cell, the foundress Varroa lays four eggs,
the first of which is a male and the other three are female. Mating occurs between
these siblings resulting in highly inbred populations. These Varroa will feed on the fat
body of the pupa and cause physical damage, induce physiological changes, and
transmit viruses. The male mite offspring dies before the bee emerges as an adult and
the foundress with two or three daughter mites escape the brood cell with the
emerging bee. In adult bees, the female Varroa will most commonly insert herself
between the abdominal sclera and puncture the intersegmental membranes to feed on
the host’s fat body. Although they are mostly present on the honey bee’s abdomen, the
mites can also be found between a bee’s head and thorax. Mites move freely between
bees in the hive and can be spread between colonies by bee drift, robbing of failing
colonies by other bees, or movement of infested bees and equipment by beekeepers.
1.2 Clinical Signs: A single mite can cause changes in a bee’s behavior, increase its
susceptibility to other diseases, and ultimately shorten its lifespan. If more than one
mite enters a brood cell to complete their reproductive life cycle, emerging bees are
weakened from feeding activity and virus transmission by Varroa. Emerging bees
show severe developmental defects, such as absence of wings, shrunken wings, loss
of hairs on the body, or shortened abdomens. Often, these highly impaired bees die in
a few days of emergence. High rates of Varroa-infested brood may result in spotty
brood patterns or large numbers of uncapped pupae that may have their heads
chewed down by worker bees. Parasitic infestation increases with increasing brood
activity and bee population growth, particularly in August and September. Early signs
of infestation regularly go undetected and are often only noticed at late stages when
damage at the colony level is apparent, characterized by clinical signs such as
scattered brood, crawling or crippled bees, supersedure of queens, and substantial
reduction of the bee population. Infestation with Varroa mites poses the risk of
additional exposure to various honey bee viruses that can be vectored by the mites,
especially variants of Deformed wing virus (DWV) and Chronic bee paralysis virus
(CBPV). Honey bees from healthy colonies may rob failing honey bee colonies of their
nectar and pollen resources. This as noted by a large amount of bee activity near the
colony that is failing due to high Varroa infestation.
2. Laboratory Criteria
2.1 Agent Isolation and Identification: Diagnosis of varroosis is based upon examination
of the hive debris, the brood, or the adult bees. Visual observation of Varroa on adult
Varroosis of Honey Bees
(Monitored)